Self-reporting fluorogenic probes

Novel self-reporting fluorogenic probes

Recently we have developed a library of fluorescent tags from aminobromomaleimide (ABM)- and dithiomaleimides (in collaboration with the Laboratory for Synthetic-Biology interactions at TAMU) which can self-report on their supramolecular state. These probes are also extremely emissive (at ca. 500 nm) in organic solvents and show non-conventional emissivity behaviour compared to standard probes. Indeed, the small size of these probes ensure that they do not disrupt the assembly process and do not change the micellar dynamics of the construct.

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We are interested in exploring this probe in a range of different application areas including;

1. Given the small size and brightness of these probes we are exploring them as tags for incorporation into polymers for applications in industry where tracking and monitroing of polymer concentration is important.

2. This new scaffold provides a method for the tracking and probing of nanoparticles and we aim to develop these probes as tags to allow for the readout of intracellular fate and assist us in exploring the role that size and morphology has in the intracellular fate of such labelled nanostructures.

Current work is focused on developing our understanding of the fluoresecent properties of these probes (in collaboration with Vas Stavros) to enable us to tailor their emission towards applications in healthcare and agriculture.